FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n01_DJ64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n01_DJ64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90538012
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCGTAT3735860.4126288966892712TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG401000.021.5339361
GATCCGC269550.021.1594311
GATCAAC1076550.020.342571
GATCCAC663550.019.2687281
GATCCGG319750.018.9721491
GATCCCG306950.018.9358881
GATCGTG449550.017.9493051
GATCCTG515100.017.8204961
GATCCGA575150.017.7766821
GATCCAG616800.017.3174631
GATCCTT1038750.016.6329461
GATCATG754950.016.2630861
GATCCCA548650.016.1752821
GATCCGT432100.016.1383781
GATCGTC605850.015.9177121
AGAGCAC4291600.015.5936888
GATCCTC756400.015.2150171
GATCGCC283150.014.9151361
GATCACC489200.014.907351
GAGCACA4513950.014.8319949
Produced by FastQC (version 0.11.5)