FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n01_DJ63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n01_DJ63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122281020
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCGTAT1655590.1353922301269649TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC397550.026.9588571
GATCACG600300.026.3149641
GATCCGG485050.025.057691
GATCGCG265550.024.4015751
GATCAAC1616400.023.9091991
GATCCAC1002000.023.8037151
GATCCTG767900.023.179581
GATCCCG462850.022.9829851
GATCCAG949800.022.9193251
GATCCGA876200.022.6333641
GATCGTG692150.022.2777561
GATCTGC736300.020.2520851
GATCCCA787150.019.6536141
GATCCTT1601550.019.532161
GATCCGT649550.019.43791
GATCATG1145050.019.3597371
GATCAGC848100.019.1395471
GATCGCA609150.018.7734761
GATCGTC972050.018.5726761
GATCACC721500.018.4951611
Produced by FastQC (version 0.11.5)