FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n01_DJ62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n01_DJ62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108603678
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTAT17545101.61551618905577TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCGCGTAT2749980.2532124188280253TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC7153200.044.6460578
GAGCACA7411300.043.2086339
GAAGAGC7745350.041.1509866
CGGAAGA7916250.040.3621984
AAGAGCA7910200.040.2365847
GGAAGAG8062600.039.6406025
TCGGAAG8076000.039.5986943
GATCGGA8446950.038.2330551
ATCGGAA8974100.035.4797442
GATCGCG223350.024.4321691
GATCAAC1398750.023.3662191
GATCACG530900.022.4024931
GATCCGC350950.021.9795151
AAGGGGG2781450.021.7878365-69
AGGGGGG2812750.020.98607365-69
GATCCGA759050.020.7452891
GATCCAC856750.020.3276561
GATCCGG412600.020.207891
AAAGGGG3002450.020.17685965-69
GCCGTCT3245100.019.97680750-54
Produced by FastQC (version 0.11.5)