FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50612411
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30555056.0370666791589915No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3395090.6708018711062786Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC745590.14731366976372653Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT6190250.023.583299
AAGAGCG6368550.022.926428
CGGAAGA6458800.022.5371325
TCGGAAG6519850.022.3252094
GAAGAGC6589350.022.183657
AGATCGG6733650.021.6633011
GGAAGAG6762900.021.5964496
ATCGGAA6733200.021.5941453
GATCGGA6786150.021.4383622
CACTCTT327500.019.5692232
GCATTCC176700.018.9509963
GATCCAC207650.016.8059351
GATCCAG210900.016.7189581
GATCCGC95450.015.9622051
TCGCCGG660400.015.77306245-49
GGATCAT373900.015.34566850-54
GATCAAC349850.015.3254091
AAAGGGG1414150.015.32483860-64
GATCCGG125000.015.2069031
CATTCCT231350.015.16358954
Produced by FastQC (version 0.11.5)