FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n02_DJ68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n02_DJ68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151443165
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9745070.6434803445899985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC478000.019.53251
GATCGCG336800.018.5454861
GATCCGG607100.018.5216061
GATCACG740050.018.3408681
GATCAAC1864150.017.6366541
GATCCAC1144650.017.4097731
GATCCTG935100.017.1684281
GATCCCG539750.017.029081
GATCCAG1146850.016.9019581
GATCCGA1075100.016.8895931
GATCGTG881800.016.6430531
GATCCTT1921350.016.3715571
GATCCTC1204400.015.4558561
GATCTGC899050.015.137571
GATCCCA927950.014.3612351
GATCCGT813350.014.1192181
GATCAGC1027950.013.9027731
GATCGCA729050.013.7417981
GATCGTC1094400.013.6154371
GATCCCT639850.013.5486771
Produced by FastQC (version 0.11.5)