FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n02_DJ67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n02_DJ67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132909264
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4578320.3444695924281095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC391250.020.5816291
GATCCGG475500.019.9557651
GATCACG605250.018.0989461
GATCCCG454300.017.4058361
GATCCTG748350.017.3814771
GATCCAC969650.017.3199711
GATCCGA856250.017.1144081
GATCAAC1513600.016.9956871
GATCGCG289800.016.7971361
GATCTGC736100.016.7246171
GATCCAG915850.016.5947551
GATCCTC1020500.016.151261
GATCCGT653250.016.147161
GATCGTG732650.016.1300471
GATCCTT1607150.015.9206281
GATCAGC812850.015.0383641
GATCGCC418500.014.4744521
GATCCCA786550.014.3145111
GATCACC705850.014.2244271
GATCCCT537500.013.7532971
Produced by FastQC (version 0.11.5)