FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n02_DJ66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n02_DJ66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135396752
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170190.30799778712564685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC1709200.019.9103011
GATCACG673000.018.540381
GATCCAC1043750.016.8407781
GATCGTG785600.016.6494351
GATCGCG324750.016.1731511
GATCCTG837500.016.1633761
GATCCGC448900.016.0150871
GATCCTT1615250.015.8765071
GATCCCG502150.015.4869751
GATCATG1247350.015.4586631
GATCCGA943250.015.4126091
GATCCGG543750.015.3961371
GATCCAG1049350.014.5248261
GATCCCA820500.014.128741
GATCCGT719650.013.2055111
GATCACA1689850.012.9647471
GATCCCT560700.012.8605961
GATCTGC801800.012.80252551
GATCCAT1575400.012.7001451
GATCCTA727800.012.5094131
Produced by FastQC (version 0.11.5)