FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50612411
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG7357601.4537145839584682Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1875010.37046446967325863Illumina Paired End PCR Primer 2 (100% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGCGTATGCCG1357240.2681634747651125Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGGGGGGGGGGGGGGGGGG1054800.20840738055335875Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGGGGGGGGGGGGGGGGGGG937250.18518185193746253Illumina Single End Adapter 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCGTAT892550.17635002608352326TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTAT837810.16553449706239048TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTAT635930.12564704732204915TruSeq Adapter, Index 15 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC250550.036.2786673
AGAGCAC2102850.032.70478
GAGCACA2374050.029.237339
GGTCGGA146550.029.0118621
AAGAGCG5360700.027.5997168
AAGAGCA2517800.027.490567
AGAGCGG5400850.027.448229
CATTCCT310100.026.7481384
GATCCGC94650.022.4600541
CGGCATT428350.021.460591
AGATCGG6898150.021.3945661
CGGAAGA7476400.019.8396935
GATCTCT317550.019.7408311
TCGGAAG7539050.019.6673534
GACCGAC80000.019.4989951
GAAGAGC7613050.019.4924627
GGAAGAG7693250.019.3077986
ATGATCT137600.019.2779439
GATCGGA7710550.019.2007372
ATCGGAA7750450.019.1118283
Produced by FastQC (version 0.11.5)