FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n01_DJ69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n01_DJ69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112355644
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTAT9184480.8174471413291886TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCGCGTAT1128210.10041418124041904TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC4812550.034.6217738
CGGAAGA5213050.032.4829754
GAGCACA5149600.032.465539
GATCGGA5445300.032.358711
TCGGAAG5297050.032.1116873
GAAGAGC5267300.031.7541186
GGAAGAG5506200.030.7293725
AAGAGCA5544900.030.2271257
ATCGGAA6153500.027.9158972
GATCCGG399850.023.1025451
GATCCGC337650.022.8666951
GATCGCG222900.022.6908741
GATCACG516150.021.5664161
GATCCTG651150.020.1709371
GATCCCG380700.020.1665381
GATCCAC849300.019.4891411
GATCAAC1372600.019.4497621
GATCCAG785450.019.2349341
GATCCGA744550.019.0927771
GATCCTC899600.018.689821
Produced by FastQC (version 0.11.5)