FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n01_DJ68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n01_DJ68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151443165
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCGTAT20044211.323546691592189TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC8520350.039.779618
GAGCACA8882550.038.1934439
GAAGAGC9204300.036.955516
CGGAAGA9285350.036.6984024
TCGGAAG9442350.036.1982083
GATCGGA9697200.036.1320271
AAGAGCA9506300.035.6564067
GGAAGAG9569050.035.5726175
ATCGGAA10622200.032.204172
GATCCGC490950.021.3430441
AAGGGGG3247800.021.20398365-69
AGGGGGG3276350.019.90487965-69
GATCACG722750.019.768971
AAAGGGG3540200.019.5063165-69
GATCGCG318550.019.476681
GCCGTCT3821150.018.94692850-54
GATCAAC1903300.018.9027821
ATGCCGT3921600.018.6968845-49
TATGCCG3913650.018.64112745-49
GTATGCC3929400.018.56456245-49
Produced by FastQC (version 0.11.5)