FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n01_DJ67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n01_DJ67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132909264
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACGGACATCTCGTAT6745280.5075101461700969TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC383450.022.481671
GATCCGG460300.019.6425531
AGAGCAC6599250.019.2830568
GATCACG583100.018.679211
GAGCACA6953450.018.2872479
GATCCCG461850.018.2097241
GATCGCG256100.017.9922311
GATCCGA853500.017.9815731
CGGAAGA7175350.017.836794
GATCGGA7566850.017.8282411
GAAGAGC7158700.017.789096
TCGGAAG7291250.017.645333
GATCAAC1524650.017.5097711
GATCCAG897600.017.2193851
AAGAGCA7433350.017.178937
GATCCAC960300.017.107651
GGAAGAG7483900.017.0577645
GATCCTG743600.016.9894871
GATCTGC723800.016.7324161
GATCGTG695350.016.6030391
Produced by FastQC (version 0.11.5)