FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l02_n01_DJ66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l02_n01_DJ66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135396752
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTAT11062960.8170772072878085TruSeq Adapter, Index 15 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCGCGTAT1459340.10778249687998424TruSeq Adapter, Index 15 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC8337400.023.8931628
GAGCACA8593050.023.2287259
GAAGAGC9209100.021.6995036
AAGAGCA9374950.021.3619277
CGGAAGA9525050.021.0030354
GGAAGAG9637950.020.7407365
TCGGAAG9774150.020.5320723
GATCAAC1720900.020.311491
GATCACG650850.020.124131
GATCGGA10316800.019.9155521
ATCGGAA10946700.018.4534192
AAGGGGG2134350.018.34887565-69
AGGGGGG2113100.017.87124465-69
GATCGCG271500.017.639851
GATCGTG726650.017.4556871
GATCCGC450100.017.2986221
GATCCAC1059150.017.1826781
GATCCGG526050.017.1184941
GATCCTG812300.016.3833871
GATCCCG492600.016.365921
Produced by FastQC (version 0.11.5)