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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2020-10-19, 19:10 based on data in: /beegfs/mk5636/logs/html/HNJW5DRXX/1


        General Statistics

        Showing 10/10 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HNJW5DRXX_l01_n01_DJ62
        50.4%
        37%
        108.6
        HNJW5DRXX_l01_n01_DJ63
        49.6%
        36%
        122.3
        HNJW5DRXX_l01_n01_DJ64
        44.8%
        36%
        90.5
        HNJW5DRXX_l01_n01_DJ65
        51.6%
        36%
        143.9
        HNJW5DRXX_l01_n01_undetermined
        49.4%
        43%
        45.2
        HNJW5DRXX_l01_n02_DJ62
        46.5%
        37%
        108.6
        HNJW5DRXX_l01_n02_DJ63
        47.7%
        36%
        122.3
        HNJW5DRXX_l01_n02_DJ64
        42.6%
        36%
        90.5
        HNJW5DRXX_l01_n02_DJ65
        48.8%
        36%
        143.9
        HNJW5DRXX_l01_n02_undetermined
        48.5%
        46%
        45.2

        Lane 1 Demultiplexing Report

        Lane 1 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 5/5 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        45,183,743
        8.9
        DJ62
        108,603,678
        21.3
        DJ63
        122,281,020
        24.0
        DJ64
        90,538,012
        17.7
        DJ65
        143,873,412
        28.2

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGGTCTTTCCC
        11680107.0
        25.9
        GGGGGGGGGGAAGCAG
        1273331.0
        2.8
        GGGGGGGGAAGGATGA
        1035248.0
        2.3
        GGGGGGGGGCTTTCCC
        931552.0
        2.1
        CCAAGTCTGCGCCTGT
        912866.0
        2.0
        GGGGGGGGGCGCCTGT
        841712.0
        1.9
        TTGGACTCATATTCAC
        837724.0
        1.9
        TTGGACTCAAGGATGA
        837225.0
        1.9
        CCAAGTCTGGAAGCAG
        834985.0
        1.9
        GGGGGGGGATATTCAC
        825943.0
        1.8
        CCAAGTCTATATTCAC
        756896.0
        1.7
        TAATACAGGGAAGCAG
        745752.0
        1.6
        CGGCGTGAAAGGATGA
        666142.0
        1.5
        TAATACAGAAGGATGA
        663282.0
        1.5
        TTGGACTCGCGCCTGT
        652817.0
        1.4
        CGGCGTGAGGAAGCAG
        634110.0
        1.4
        CGGCGTGAATATTCAC
        483907.0
        1.1
        TAATACAGGCGCCTGT
        460092.0
        1.0
        CGGCGTGACAGACTCT
        291815.0
        0.7
        CCAAGTCTAAAAAAAA
        286367.0
        0.6

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        1.0
        1,276,674,048
        510,479,865
        8.8
        2.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (151bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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