FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45183743
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30741936.803759042273236No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4156430.9198950162229809Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC719600.15926082086647847Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT5716700.023.9713959
GCATTCC181750.023.8496063
AAGAGCG5890550.023.2836068
CGGAAGA6056400.022.622145
TCGGAAG6093050.022.4752924
GAAGAGC6140450.022.3619967
GGAAGAG6287300.021.8341566
AGATCGG6321150.021.7260151
ATCGGAA6324100.021.6755543
GATCGGA6366900.021.5382162
GATCAAC350650.019.184311
GGATCCC29550.019.1327615
GATCCAC206600.018.79161
CATTCCT234700.018.7163734
GATCCGC94350.018.6898021
GATCCCG95800.017.5736831
GATCACG139400.017.4910871
GGGATCC32050.017.4150544
GATCCGG112200.017.2686631
GATCGTG161000.016.3163491
Produced by FastQC (version 0.11.5)