FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n02_DJ65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n02_DJ65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143873412
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2446040.17001334478673516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC1725100.019.0329531
GATCACG658600.018.9720081
GATCCGC438800.017.5283531
GATCGTG774050.017.5203251
GATCGCG297250.017.038661
GATCCAC1066550.016.103451
GATCCGA950950.016.0771391
GATCCGG533900.016.0642131
GATCCTT1643650.015.7204331
GATCCTG817050.015.7101771
GATCCAG1030300.015.5643321
GATCATG1243700.014.8365391
GATCCCG496550.014.7883391
GATCGCA654700.013.488081
GATCCCA855000.013.2815911
GATCTGC794500.013.2518171
GATCACA1697100.013.1046341
GATCCGT706750.013.09019851
GATCAGC905000.012.9567071
GATCGTC1093000.012.7064331
Produced by FastQC (version 0.11.5)