FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n02_DJ64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n02_DJ64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90538012
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG999280.11037132116397697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC271500.020.9269431
GATCAAC1075400.020.5461831
GATCACG418900.020.5441511
GATCCAC657050.018.840591
GATCCGG336300.018.534471
GATCGTG474050.018.5214541
GATCGCG204450.018.2782231
GATCCGA587000.018.1839581
GATCCCG311400.017.802811
GATCCTG522300.017.5606611
GATCCAG639500.017.19041
GATCCTT1038600.017.0193441
GATCCGT458750.016.1338481
GATCCCA515200.015.6900091
GATCATG785550.015.0658621
GATCTGC479400.015.0150761
GATCCTC718850.014.747751
GATCGTC636000.014.5125541
GATCGCC274300.014.2585971
GATCAGC566300.014.222971
Produced by FastQC (version 0.11.5)