FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n02_DJ63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n02_DJ63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122281020
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1393650.1139710807122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC404500.026.4061451
GATCACG618100.026.0738741
GATCCGG488950.024.8728331
GATCAAC1592750.023.6174561
GATCCAC994900.023.2808691
GATCGCG280800.023.0506651
GATCCGA877550.022.9873371
GATCCTG783050.022.5922741
GATCGTG719450.022.2091731
GATCCCG458100.022.0809731
GATCCAG947250.022.0466161
GATCTGC758150.020.7787971
GATCCTT1585200.020.0129831
GATCGCA602400.019.3212471
GATCCGT666700.019.112161
GATCATG1165400.019.0965331
GATCAGC836850.018.781341
GATCGCC433200.018.7605381
GATCCCA774200.018.6796991
GATCCCT540500.017.5332811
Produced by FastQC (version 0.11.5)