FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n02_DJ62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n02_DJ62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108603678
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2265570.20860895705576382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG541200.024.2141651
GATCAAC1394700.022.2103211
GATCCGC349150.021.9670851
GATCCGG428550.021.2157841
GATCCAC851300.020.8318611
GATCGCG249050.020.5114141
GATCCGA745250.019.9016191
GATCGTG620650.019.793371
GATCCTG667500.019.6107751
GATCCAG811550.019.3308051
GATCCCG392700.019.272271
GATCCTT1362100.018.4321751
GATCCCA656950.018.0480061
GATCATG1024400.017.6021751
GATCTGC618550.016.9630131
GATCCGT574450.016.5473541
GATCGCA518100.016.2602831
GATCAGC721800.016.0344221
GATCACA1374050.015.9113161
GATCCAT1295550.015.3743821
Produced by FastQC (version 0.11.5)