FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45183743
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG7252361.6050817215386517Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGGGGGGGGGGGGGGGGGG2022200.4475503501336753Illumina Single End Adapter 1 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1989860.4403929085733335Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGGGGGGGGGGGGGGGGGGG1373070.30388584673031627Illumina Single End Adapter 1 (96% over 25bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGCGTATGCCG1115950.2469804239104317Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTAT979690.21682355974802706TruSeq Adapter, Index 2 (97% over 36bp)
AATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGGGGGGGGGGGGGGGGGG502630.11124133739871883Illumina Paired End PCR Primer 2 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA122950.031.0983581
AGAGCGG4831550.028.8480729
AAGAGCG4817900.028.8245168
GATCACG143200.025.6367471
AGAGCAC1986250.024.5389868
GCATTCC197900.023.3503423
GAGCACA2202050.022.3085889
AGATCGG6434350.021.4388451
GATCCTG166950.020.946721
GATCCGC90150.020.9248811
AAGAGCA2353350.020.7547557
GATCAAC359400.020.5102541
CGGAAGA6858800.020.2336645
TCGGAAG6899750.020.0967854
GAAGAGC6938100.020.0604487
CGTAGGA82050.019.7895875
GATCCAG208650.019.7856031
GGAAGAG7035250.019.7684296
ATCGGAA7120150.019.4773643
GATCGGA7099000.019.4056342
Produced by FastQC (version 0.11.5)