FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n01_DJ65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n01_DJ65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143873412
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTAT4388080.3049958945854429TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG648200.019.7471641
GATCAAC1725500.019.727211
GATCGCG276750.018.589761
GATCGTG740000.018.3368641
GATCCGC431550.017.991521
GATCCGG507800.016.961851
GATCCAC1047800.016.4544431
GATCCGA920150.016.2214931
GATCCTG818950.015.709651
GATCCTT1667200.015.2377051
GATCCCG500900.015.19636251
GATCCAG998300.015.1914911
GATCATG1231950.014.8371431
GATCCCA861100.013.735651
GATCCGT710800.013.7204081
GATCCCT573050.013.6502761
GATCGTC1048650.013.2995821
GATCACA1705500.013.1043091
GATCGCA648350.012.881931
GATCAGC900150.012.8012031
Produced by FastQC (version 0.11.5)