FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n01_DJ64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n01_DJ64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90538012
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCGTAT3735860.4126288966892712TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG400400.021.4938051
GATCCGC269700.021.0670381
GATCAAC1076450.020.4254421
GATCCAC664600.019.2056141
GATCCCG306450.018.9905451
GATCCGG320500.018.837271
GATCGTG450750.017.9820861
GATCCTG514700.017.7357331
GATCCGA574300.017.6514451
GATCCAG618850.017.2249931
GATCCTT1037750.016.607091
GATCATG753700.016.2323631
GATCCGT432700.016.1831511
GATCCCA548550.016.17831
GATCGTC607150.015.8239411
AGAGCAC4296050.015.5724728
GATCCTC756200.015.1519361
GATCACC489450.014.9739271
GATCGCC282650.014.8388941
GAGCACA4518600.014.81512459
Produced by FastQC (version 0.11.5)