FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n01_DJ63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n01_DJ63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122281020
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCGTAT1655590.1353922301269649TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC399900.026.7098261
GATCACG600750.026.3919981
GATCCGG482750.025.011841
GATCGCG266700.024.4052961
GATCCAC999550.023.8839441
GATCAAC1616350.023.8067911
GATCCTG768000.023.1105271
GATCCAG952000.022.9198151
GATCCCG461200.022.8293081
GATCCGA873950.022.5837971
GATCGTG689700.022.230741
GATCTGC735600.020.2221241
GATCCCA784850.019.7112961
GATCCTT1600700.019.687681
GATCCGT648950.019.4335981
GATCATG1145250.019.3501071
GATCAGC849900.019.2271611
GATCGTC970000.018.5820871
GATCGCA608350.018.5716171
GATCACC719950.018.4443511
Produced by FastQC (version 0.11.5)