FastQCFastQC Report
Mon 19 Oct 2020
HNJW5DRXX_l01_n01_DJ62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_l01_n01_DJ62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108603678
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTAT17545101.61551618905577TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCGCGTAT2749980.2532124188280253TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC7157250.044.625858
GAGCACA7412850.043.2074139
GAAGAGC7748500.041.144726
CGGAAGA7922000.040.346794
AAGAGCA7912900.040.2329257
GGAAGAG8069150.039.6211625
TCGGAAG8076600.039.6048933
GATCGGA8447500.038.249621
ATCGGAA8974650.035.4938772
GATCGCG223000.024.5356941
GATCAAC1401350.023.286711
GATCACG528250.022.6523381
GATCCGC351050.021.8493181
AAGGGGG2782000.021.78247565-69
AGGGGGG2814000.020.98035265-69
GATCCGA759200.020.7221551
GATCCAC858300.020.3840261
GATCCGG412800.020.1805971
AAAGGGG3004350.020.1592765-69
GCCGTCT3243850.019.99299250-54
Produced by FastQC (version 0.11.5)