FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9846353
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA12450.070.91982
TAGTAAC75400.069.4506762
TAACAAG75700.068.095985
AAACACG33350.063.3344735
CACGCAC16350.054.0078773
ACACGAG26500.052.536517
AACACGT13950.051.6009866
CGCACTT18100.051.0312165
ACGCACT19650.049.7716754
CACGAGC28800.046.5097668
ACACGTG16000.044.5679367
ACGAGCC10900.038.8605659
GTAACAA141600.037.0764854
AGCGGCC5800.035.14949145
GTCGCCC10150.033.93744145
ACAACGC14450.033.1406178
TCTCGGG13900.032.873623145
GTCGGCC3050.032.268383145
AACAACG16450.031.7890857
GCACGCC6150.030.862967145
Produced by FastQC (version 0.11.5)