FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_POSITIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_POSITIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952764
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT128710.4358966717285906No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT123390.4178796544525739No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC110210.3732435101484575No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT84140.2849533521812106No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT76320.2584696914484192No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT66050.22368872012798854No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT57680.19534239783470675No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA53530.18128776969646068No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT52520.17786724573992369No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC50290.1703149997764806No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT46240.15659903737650555No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG43550.14748892901701594No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG42850.145118268849119No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG37860.1282188485093966No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT37660.12754151703285463No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT36570.12385006048570085No Hit
GTATTATTATCACTATATGTGTCAGCTTTATTATCATACTTACTATATTC34600.11717834544176235No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT33830.11457061925707573No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT33690.11409648722349637No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT33120.11216609251535171No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32700.11074369641461355No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG31540.10681517385067009No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATTGTTCAAACA31510.10671357412918878No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG31250.10583304320968422No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC30990.10495251229017963No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT30970.10488477914252545No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC30480.1032253170249976No Hit
GTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAATG30180.10220931981018463No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA29650.10041439139734838No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGGA100.008178558138.245134
ACGGCGC100.008183114138.219396
GTAACAA67300.0121.2982944
AGCACGC19450.0120.136731
CACGCAC20000.0118.20963
GCACGCA19850.0118.064112
GAAACAC20350.0111.071644
AAACACG21650.0104.719715
TAACAAG83350.098.022195
GAGCAGA4450.097.872181
ACGCACT26000.091.720334
CGCACTT25950.091.6291355
TAGAAAC26900.088.406642
TAGTAAC94700.087.017182
GAGCCCC2000.084.254265145
TTCGGAA1700.082.60221145
GAAGCGG6900.082.156791
ACGTGCA11350.077.963772
AGTAACA105850.077.912343
TTACCCC1850.072.1095145
Produced by FastQC (version 0.11.5)