FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_NEGATIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_NEGATIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAG270.7311129163281885No Hit
GTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGCAGAA210.5686433793663688No Hit
GTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT110.29786081776333606No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTCTT100.27078256160303277No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT90.2437043054427295No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCTTATACACATCTGA80.2166260492824262No Hit
TGTTTCTACTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTT80.2166260492824262No Hit
CTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTTGTTACTAC80.2166260492824262No Hit
GTAGAAACACGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT70.18954779312212292No Hit
AACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAGTAGAA70.18954779312212292No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC60.16246953696181965No Hit
TATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTT60.16246953696181965No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTC60.16246953696181965No Hit
AGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTGAC60.16246953696181965No Hit
TGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATCTGTCTCTTATA50.13539128080151638No Hit
ATTAGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGT50.13539128080151638No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTTATAC50.13539128080151638No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTCTT50.13539128080151638No Hit
ATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTA50.13539128080151638No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTGA50.13539128080151638No Hit
GTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGCTGTCTCTT50.13539128080151638No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTC50.13539128080151638No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTCTT50.13539128080151638No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTC50.13539128080151638No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT50.13539128080151638No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCTGTCTCTTATACACATCTGAC50.13539128080151638No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT40.1083130246412131No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC40.1083130246412131No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGT40.1083130246412131No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTC40.1083130246412131No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCGTGCTCTGTCT40.1083130246412131No Hit
AGCAGAAGCAGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTGAC40.1083130246412131No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTGTCTCT40.1083130246412131No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGT40.1083130246412131No Hit
GAGCAGAAGCACGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACTA40.1083130246412131No Hit
AACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAGTAGTA40.1083130246412131No Hit
GCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTCTTATA40.1083130246412131No Hit
CACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCTTAT40.1083130246412131No Hit
GCAGAAGCAGGCACGTGTTTCTACTAATAACCCGGGCTCTTGTTACTACT40.1083130246412131No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCTGCTTCTGCT40.1083130246412131No Hit
GAGCAGAAGCGGAGCACGTGTTTCTACTAATAACCCGGGCTCTTGTTACT40.1083130246412131No Hit
AGCAGAAGCGGAGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACTA40.1083130246412131No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC40.1083130246412131No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGT40.1083130246412131No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTCTTAT40.1083130246412131No Hit
GTAGAAACACGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA40.1083130246412131No Hit
GAGCAGAAGCGGAGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACT40.1083130246412131No Hit
TATTAGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGG40.1083130246412131No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTACCTGTCTC40.1083130246412131No Hit
TAGTAGAAACACGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTA40.1083130246412131No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGTGTTTCTACCTGTCTCTTATAC40.1083130246412131No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGCG407.151614E-569.534245
GAAGCGG502.1582861E-455.6274036
GCGGAGC502.1582861E-455.6274039
AGCGGAG502.1582861E-455.6274038
AAGCGGA502.1582861E-455.6274037
AACACGT400.00658405852.1506846
GAAACAC1004.4594344E-848.6739774
TAGAAAC1109.450741E-844.2490652
AAACACG1109.450741E-844.2490655
GTAGAAA1151.3410317E-742.325191
AGAAACA1201.8742685E-740.5616463
AACACGA750.001593882537.084936
ACACGAG800.002188661634.767127
CACGAGC800.002188661634.767128
GAGCAGA850.002947149632.7221
AGCAGAA900.003900310530.904112
GCGGGGG500.001751102527.8137023
AGCGGGG500.001751102527.8137022
GCAGAAG1050.0082903326.4892373
GGGAGCA600.005900686623.1780838
Produced by FastQC (version 0.11.5)