FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_DEMUX_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_DEMUX_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTAT481.4883720930232558No Hit
ATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG220.6821705426356589No Hit
GTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC210.6511627906976745No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC210.6511627906976745No Hit
GTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACA200.6201550387596899No Hit
ACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGG190.5891472868217054No Hit
ATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGT190.5891472868217054No Hit
GTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC190.5891472868217054No Hit
ATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATC180.5581395348837209No Hit
GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCT170.5271317829457364No Hit
GTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACC160.496124031007752No Hit
GGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG140.4341085271317829No Hit
GCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG130.40310077519379844No Hit
AAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA120.37209302325581395No Hit
CCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCA110.34108527131782945No Hit
GGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGAC110.34108527131782945No Hit
GTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACA110.34108527131782945No Hit
CCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGT100.31007751937984496No Hit
ATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG100.31007751937984496No Hit
CCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGG90.27906976744186046No Hit
TCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTT90.27906976744186046No Hit
AGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT90.27906976744186046No Hit
GAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCG90.27906976744186046No Hit
CCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGG80.248062015503876No Hit
CCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCC80.248062015503876No Hit
ATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATT80.248062015503876No Hit
CGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCT80.248062015503876No Hit
ACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGC80.248062015503876No Hit
TGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGA70.21705426356589144No Hit
AAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA70.21705426356589144No Hit
GCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG70.21705426356589144No Hit
TTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGT70.21705426356589144No Hit
AGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGAC70.21705426356589144No Hit
CGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGG70.21705426356589144No Hit
GCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG60.18604651162790697No Hit
TACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTC60.18604651162790697No Hit
ATTAGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTG60.18604651162790697No Hit
CCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTG60.18604651162790697No Hit
GCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACC60.18604651162790697No Hit
GTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGAT60.18604651162790697No Hit
AACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGG60.18604651162790697No Hit
GTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAA60.18604651162790697No Hit
ACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGG60.18604651162790697No Hit
GAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTC50.15503875968992248No Hit
GAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGA50.15503875968992248No Hit
GGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG50.15503875968992248No Hit
GGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGG50.15503875968992248No Hit
GAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCA50.15503875968992248No Hit
TATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCT50.15503875968992248No Hit
AGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGT50.15503875968992248No Hit
CTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGA50.15503875968992248No Hit
GAAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC50.15503875968992248No Hit
CCTGTATCCATGTCCCCGAAGGGAACGTCTAATCTCTTAGATTTGCATAG40.124031007751938No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT40.124031007751938No Hit
GAGCAGAAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACC40.124031007751938No Hit
GAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC40.124031007751938No Hit
AACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG40.124031007751938No Hit
ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATC40.124031007751938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2403.3051E-69.46418130-134
Produced by FastQC (version 0.11.5)