FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_99_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_99_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606493
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA36200.078.681931
TAGTAAC40550.069.3888852
ACACGAG20250.067.5748147
AACACGA20550.066.924626
AAACACG33250.065.2716145
CACGAGC22600.060.1188628
ACACGTG13450.058.5770157
GTAACAA48200.058.22024
CGACGAA7050.056.756435145
CGGAGCG755.235288E-755.2937935
AGTAACA51150.055.274193
ACGAGCC9150.054.5810669
GCCGACC3250.051.839077145
ACGAGCA10650.051.452699
ACGAGCG1102.4811015E-950.4461339
AACACGT15700.050.182226
TAACAAG57300.049.6968655
ACGAGCT5500.046.6626789
TGACGCC6700.044.005188145
GTAGAAA65800.043.497151
Produced by FastQC (version 0.11.5)