FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_95_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_95_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188398
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA59690.14251272204790472No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG46370.1107105867207462No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA42680.10190053571795231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC94100.078.949952
GTAGTAA98850.076.203971
AAACACG60600.071.038115
GCACGCA30250.070.3652652
GTAACAA106600.069.1004944
AGCACGC33600.063.5546041
AGTAACA118700.062.5252533
ACACGAG45550.060.2187087
ACAACGC16700.058.9411968
TAACAAG125400.058.6851235
AACACGA47100.058.6770636
CGCACTT33350.057.807525
ACGAGCG3250.057.6156929
CACGCAC37650.057.2652933
GAAACAC79050.054.7209474
CACGAGC50200.053.9908458
AACACGT28950.053.4599766
TGACGCC12750.053.427303145
ACGCACT42250.052.9906774
CGACGAA12450.052.45843145
Produced by FastQC (version 0.11.5)