FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_92_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_92_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861699
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG14950.1734944568811151No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT13810.16026477923265547No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT12920.14993634668254227No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA12580.14599065334879116No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA11970.13891161530882595No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT11700.1357782706026118No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA11380.1320646768767284No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT10320.11976339765973965No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA10310.1196473478558058No Hit
CTGTAGGACATTTGTCCATTGAAGGCATCAAAGAAGCAGATATAACCCCA9980.11581770432598855No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA9540.11071151295289888No Hit
GTACATGATGGTGGGAAAACGACTTGGCACTCAGCAGCAACAGCCATTTA8890.10316827569719822No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC8690.1008472796185211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC2350.080.64222145
ACACGAG5700.072.699447
TAGTAAC9950.065.948612
AAACACG8500.065.005625
CAACGCA4150.060.1031729
AACACGA6850.059.4861836
GTAGTAA11450.057.9123041
CACGAGC6750.057.4412578
ACAACGC4350.057.2997788
TGACGCC4600.054.930202145
ACGAGCC4950.054.588659
AGTAACA12050.053.8791473
GTAACAA12200.052.6505624
GAAACAC10700.050.9944844
ACGTGCC1800.050.03969
TAACAAG12900.049.7935525
TAGAAAC13200.047.6181532
ATTAGTA5100.047.4026831
TCGCCGG7150.046.13796145
TCTCGGG4200.045.12124145
Produced by FastQC (version 0.11.5)