FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_91_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_91_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928752
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT52000.5598911227109067No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35800.3854635037125088No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT28770.30977053077678435No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20330.2188958947060141No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA19830.2135123262184092No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT19380.20866711457956486No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG18980.20436025978948094No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA17590.1893939393939394No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT14240.15332403052698676No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12480.1343738694506176No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA12130.1306053715092942No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG11610.12500646028218512No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA11370.12242234740813479No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT11120.11973056316433234No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG10900.11736179302978621No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT10320.11111685358416457No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT9860.10616397057556808No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC9850.10605629920581597No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA9680.10422588592003032No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC9600.10336451496201354No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT9540.10271848674350097No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT9510.10239547263424466No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA9480.10207245852498836No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG9440.10164177304597997No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG9430.10153410167622788No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT9310.10024204523920273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTC100.0078107836140.38422145
AGCACGC8650.0117.6092761
GCACGCA8950.0113.667072
CACGCAC9150.0111.926833
GAAACAC5850.096.9850544
AAACACG5850.095.802315
GTAACAA21950.093.30484
ACGCACT11850.087.5829854
CGCACTT11850.087.5829855
GAAGCGG2250.079.970761
GGCACCC451.4942834E-677.99124145
TAACAAG26800.077.193955
GTCGCCC757.4578566E-1174.87159145
AGAAGCG853.6379788E-1273.260595
GCCGACC1250.072.999794145
TCTCGGG1250.072.999794145
ACGTGCA2600.071.867212
TAGTAAC28550.071.750662
GGCCCCC300.00220070970.19211145
AACACGA6750.067.6493456
Produced by FastQC (version 0.11.5)