FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_88_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_88_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456009
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30010.20611136332261684No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27290.18743016011576852No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT25840.17747143046505895No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT22450.15418860735064138No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA22430.15405124556235572No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT22180.15233422320878512No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22170.1522655423146423No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT21690.1489688593957867No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21590.14828205045435847No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20960.14395515412336052No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC20340.13969693868650537No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC19650.13495795699065047No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC18830.12932612367093885No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT18080.12417505661022699No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG16960.11648279646623064No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT16530.11352951801808918No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA16480.11318611354737505No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT16310.11201853834694703No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA16220.1114004102996616No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT16010.10995811152266229No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT15720.10796636559252037No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA15240.10466968267366479No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA14770.10144168064895204No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14690.10089223349580945No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC14680.10082355260166662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC1050.0111.753973
GTAACAA23850.0107.067344
GGATCCC204.3876987E-4105.346344145
TTCGGAA406.008122E-9105.346344145
GCACGCA1050.098.6064452
AGCACGC1050.098.6064451
GAAACAC9050.095.324624
AAACACG9400.092.5063255
TAACAAG27850.091.686485
GACGACC250.001065138784.277084145
AACACGA9300.075.68356
ACACGAG9400.074.878367
TAGTAAC35250.073.234512
GAGCAGA3000.071.325331
AACACGT2350.070.473756
GTCGCCC1300.070.230896145
TCTGACC1700.070.23089145
AGTAACA38950.066.277713
CACGAGC10500.064.63168
TAGAAAC14800.063.4279332
Produced by FastQC (version 0.11.5)