FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_86_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_86_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561466
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23410.14992321318555768No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT21910.14031685608268127No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT21280.13628218609947318No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC19790.12673987137728263No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC19730.1263556170931676No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC19020.12180860806447275No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT18580.11899074331429567No Hit
CTTTACATATAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGGG17770.11380331047874241No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT17170.10996076763759184No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17130.10970459811484848No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA16590.10624630955781299No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA16430.1052216314668395No Hit
TCCCAATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAG16350.10470929242135274No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA16270.10419695337586601No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA16190.10368461433037927No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG16110.10317227528489252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1550.084.709172
AGCACGC1700.077.234831
CGCACCC555.4993507E-876.57687145
CACGCAC1800.072.932333
GCGGTGC408.451351E-569.104851
GACGAAA8550.067.32197145
AACACGA6000.063.3197446
AAACACG8750.063.1633725
GTACCCC451.4009903E-462.395973145
ACGAGCT350.004254796559.436469
GCCGACC1300.059.396168145
GTAACAA23400.059.0469474
CACGAGC6350.058.9343578
ACGAGCC2600.058.6744549
TGACGCC3750.058.02826145
GAAACAC9300.057.1993524
ACACGAG6900.056.0635457
AGAAGCG4100.055.6049425
ACGTGCA1500.055.4740339
CGTTTTT1051.4788384E-953.482258145
Produced by FastQC (version 0.11.5)