FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_85_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_85_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563441
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG34770.13563799595933745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA54800.094.354341
TAGTAAC58350.088.376912
AGCACGC19400.080.17181
GTAACAA67700.075.853084
AGTAACA68050.075.2656863
GCACGCA20500.074.5210952
TAACAAG74200.069.4836355
AAACACG39500.067.711355
CGCACTT23100.063.7257965
ACGCACT29150.060.2238044
CACGCAC27900.057.228813
AACACGT16650.056.446686
TCGCCGC1750.056.167637145
AAGAGCG14100.056.0673569
ACGGCGA755.1356074E-755.447878
GAAACAC50500.052.9654464
CAAGAGC94750.051.4246068
ACAAGAG101100.050.7868657
ACACGAG34550.050.204137
AACAAGA102650.050.1546336
Produced by FastQC (version 0.11.5)