FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_84_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_84_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243702
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG16340.13138195484127227No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT15250.12261779751097932No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG14960.12028604923044267No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA14230.11441647597254005No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC14050.11296918393634486No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT13550.10894892828024719No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG100.007806874140.40965145
ACGAGCG555.9299055E-1088.2338949
AAACACG6600.080.585155
ACACGAG5000.075.9802867
AACACGT2000.072.526656
CACGAGC5400.070.584828
GAAACAC7650.070.427374
GCCGACC1850.068.3074145
TAGTAAC17200.065.870882
AACACGA5900.065.56086
GTAACAA18650.060.739794
TATCGGG350.00405137760.175564145
TAACAAG19200.058.9998445
ACGAGCA4100.055.799489
ACACGTG2600.055.7897227
ACGTGCG502.5080648E-455.4613049
ACGAGCC1251.8189894E-1255.4613049
GAGCGTT502.5538937E-455.2583967
TCTCGGG6800.054.718464145
GTCGCCC3000.053.823696145
Produced by FastQC (version 0.11.5)