FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_81_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_81_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774637
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT62890.35438233283764514No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT52760.2973002366117691No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA45030.25374203287770963No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT32300.18200905311903223No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA29760.1676962668985263No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA28370.1598636791636825No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC27550.15524301589564513No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA26390.14870646785793376No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG25530.14386040638169947No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA25200.14200087116407467No Hit
GGCTTGGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGA24740.13940879176980983No Hit
TGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCTA24550.13833815028087434No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC23400.1318579517952122No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC23110.13022381478578438No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC22700.1279134831517657No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG21870.1232364703316791No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG21800.12284202346733444No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA21580.12160233332225126No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG21420.12070074048946347No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT20460.11529118349273683No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT19500.10988162649601017No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC19260.1085292372468285No Hit
CCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATT18730.105542710988219No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT18660.10514826412387433No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG18600.10481016681157893No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA18300.10311968025010186No Hit
GTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGT18250.10283793248985566No Hit
GGTCAGTACAGTGGGTTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA18040.10165459189682173No Hit
GTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCT17850.10058395040788623No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT17810.1003585521996893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA11300.082.9595649
GTAACAA16600.072.920384
GCCGACC2750.071.466995145
CGCGACC300.002201207770.1908145
CGCCGGG407.823588E-570.1908145
AACAACG13700.069.667237
TCCGACC451.4015049E-462.391823145
TGACGCC6650.062.274548145
ACAACGC15600.061.839788
GCTGCCC6400.061.41695145
TAACAAG20150.060.414945
TGGCTAG350.00429750859.2870645
GACGAAA13550.058.017487145
GTCGCCC3650.057.691067145
TAGTAAC22050.056.1612632
CACCGTT502.5379233E-455.3299187
AGTAACA24200.050.591423
GAAGCGG6700.048.530581
TCTCGGG10600.047.014595145
GCACCGT606.2396855E-446.108266
Produced by FastQC (version 0.11.5)