FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_80_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_80_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092946
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC15700.082.750292
TCGATCC354.0339834E-580.22589145
GTAGTAA16850.077.922881
AGTAACA17550.073.226393
GTAACAA20450.063.1742864
CTTAGCG350.004258907459.4207579
AACACGT4550.057.6905446
AAACACG13450.054.9583475
AACACGA8800.054.9474836
ACAACGC4550.054.819738
GAAACAC14100.052.9171834
AACAACG5000.049.735327
ACACGAG9650.047.9600837
GAAGCGG11200.047.5098841
CACGAGC9750.046.9013258
CAACGCA5700.046.216159
TAACAAG27600.046.055445
AAGCGGA11450.045.8690152
TGACGCC5750.045.170666145
GACGAAA6750.044.71851145
Produced by FastQC (version 0.11.5)