FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_78_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_78_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30070.24331116803925343No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA21510.1740479954946572No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20130.16288173636947695No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16860.1364225571380716No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA15610.12630819198845183No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT15270.12355708466775524No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG14700.11894493415952863No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG12910.10446116326527309No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC12840.10389475881689438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA9050.097.738322
TAGTAAC40550.092.365952
GTAACAA41400.089.7764364
AGCACGC9950.088.897671
GTAGTAA42550.088.8364641
CGCACTT11100.085.262965
AGTAACA46250.080.972633
TAACAAG46400.080.2478945
ACGCACT11950.079.201444
CACGCAC11150.078.7008743
AAACACG15800.077.388975
GAAGCGG4500.069.104041
AACACGT6050.066.2216956
GAAACAC18850.065.236274
TGACGCC3700.062.627018145
GACGAAA3550.061.31727145
CAAGAGC56300.060.302498
ACAAGAG62450.059.40257
TAGAAAC27150.058.7957042
CTCGCTC9050.057.2615933
Produced by FastQC (version 0.11.5)