FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_77_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_77_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557153
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT68260.43836411707776946No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45370.29136507459446825No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT39210.25180569924728013No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA30400.19522808612898027No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30320.1947143280075882No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26110.1676778068693314No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22520.14462291117186302No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA22260.14295319727733882No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21970.14109082408729265No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT21280.13665966029028617No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC19010.12208177359578666No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18320.1176506097987802No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG17680.11354054482764378No Hit
GTAAGAAGTACAGTAAAAATGAATTTAATGCGATTTTTCTACCTCCAAAA17430.11193505069829361No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC17200.11045799609929147No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT16460.10570573347641497No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT16200.10403601958189079No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT16020.10288006380875868No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT16010.10281584404358468No Hit
GTAATAAAAGGGTCCTTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA15960.10249474521771465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC151.4900284E-4138.187621
GAGCGGT100.008194662138.152117
AGCACGC10050.0118.250111
CACGCAC10300.0116.039583
GCACGCA10350.0115.4901352
ACTCGCC257.5794487E-6112.35085145
CGCACCC257.5794487E-6112.35085145
TCGCGCC204.3906452E-4105.32892145
AAACACG7850.0104.7274865
GAAACAC7950.0104.2791444
GGCACCC950.096.08955145
GCTGCCC2000.091.285065145
ACACGTG2150.086.746677
GTAACAA31400.086.246164
AACACGT2200.084.772446
GGCCCCC250.001065853484.26314145
CGCACTT14450.083.1887745
CGAGGAT250.001137072682.901925
ACGCACT14750.081.965174
ACAACGC4150.080.170668
Produced by FastQC (version 0.11.5)