FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_75_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_75_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178026
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT23920.10982421697445302No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC22770.10454420654298893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA29550.081.62861
TAGTAAC29250.081.284362
AGCACGC19000.075.663111
ACGCACT21150.069.2664
GCACGCA20750.068.615712
CGCACTT16600.068.270075
TAACAAG35100.065.755795
GTAACAA38850.061.0095374
AGTAACA39250.060.393333
CGACGAA3450.054.930355145
AAACACG26200.052.7501075
ACACGAG19750.049.330647
CACGCAC29750.049.247413
AACACGA20450.047.9799466
GTAGAAA38750.045.482351
TCTGTCG20000.044.365248
TCGCTCT20050.042.7367974
TAGAAAC41750.042.3797042
CACGAGC23150.041.9217958
CGCACTG4950.041.880395
Produced by FastQC (version 0.11.5)