FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_73_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_73_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928706
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC42680.22128826270048416No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG38940.2018970231854933No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36630.18992008113211656No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA33740.17493594150689634No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31590.16378857119747645No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29530.15310783499403227No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG29170.1512412985701294No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT25600.13273147903309265No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC24600.12754665563336245No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23300.12080638521371324No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA22400.11614004415395607No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC22120.11468829360203163No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG21870.11339208775209908No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA21700.11251066777414494No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT20810.10789617494838509No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT20520.10639257616246332No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA20490.10623703146047142No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC20210.10478528090854698No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT19810.10271135154865489No Hit
TCCCTATAATGCACGACAGAACAAAAATCAGGCAACTAGCCAATCTTCTC19420.10068927042276013No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA19300.1000670916147925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC100.0077968347140.47212145
AGCACGC4950.0107.4540561
GCACGCA5000.0106.379522
CACGCAC5250.0101.300683
GAAACAC8700.0100.816514
AAACACG9150.095.8558355
AACACGT2250.082.867346
GGCACCC850.082.63067145
GCTGCCC3250.082.12217145
ACACGTG2350.079.3410647
ACACGAG8900.078.367057
AACACGA9050.077.068156
ACGAGCC2200.072.442949
TCCGACC502.7854512E-670.23607145
AAGCGCA300.002195616570.23606145
GCCGACC1200.070.23606145
CACGAGC10000.067.8667768
TAACAAG47100.065.542435
ACAACGC4550.063.9247478
CGTGCTC451.3970729E-462.432056145
Produced by FastQC (version 0.11.5)