FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_6_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_6_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190218
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT35400.16162774664439794No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA34190.15610318242293691No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33150.15135479664581333No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC33040.15085256353477142No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32820.14984809731268758No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA31180.14236025820260814No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG29860.13633346087010517No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT29830.13619648820345737No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC28370.1295304850932647No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG27780.12683668931585804No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT26990.12322974242746612No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT26980.12318408487191686No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT25680.11724860265051242No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG25170.1149200673174999No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC24730.11291113487333225No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT24610.11236324420674107No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA24520.11195232620679768No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG24190.11044562687367193No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT23870.10898458509609546No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23680.10811709154065943No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC23640.10793446131846236No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC23580.10766051598516677No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC23420.10692999509637853No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC23160.10574289865209764No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC22790.10405356909677485No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT22650.10341436331908513No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21960.10026399198618587No Hit
AATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTCCTTA21920.1000813617639888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA100.0078067672140.41275145
ACACGAG5650.084.3802957
ACGAGTC250.001066690784.24766145
GTAACAA30350.080.8312764
CACGAGC6200.076.042688
AAACACG9150.074.767695
ACGAGCC2250.073.980659
AACACGA6650.071.691526
TAACAAG34300.071.521085
ACGAGCG408.3011146E-569.356869
TAGTAAC38200.065.491472
ACACGTG3100.064.636337
AACACGT3250.063.7790836
TGACGCC6600.062.76025145
GTCGCCC5500.061.271023145
AGTAACA40750.061.2208373
CGGCGTT350.004051439560.176903145
CGTACGA350.004051439560.176903145
GCCGACC4600.059.5228145
GGCGCCC851.6836566E-857.817024145
Produced by FastQC (version 0.11.5)