FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_68_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_68_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116551
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA140680.34174239551508045No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC99980.2428732208103337No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA95170.23118868198159093No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT68360.16606134601514713No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT67710.16448235428153324No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA65870.16001259306638008No Hit
AGTAGAAACAACGCACTTAGCCGATAAAGAATTTTTTGGCTAGAGGGCTT65480.15906519802621175No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA64770.15734045320949505No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA60290.14645755633781776No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG60120.14604458926902641No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT58950.14320240414852142No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC57660.14006871286181077No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG57210.13897556473853961No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC51790.12580920289825145No Hit
CCCTTAGACAGTGCCGTTCAATGTTTTCAAATCTTGTTACTATTACTCTT47790.11609233069139674No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG46410.11274000978003187No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC42290.10273163140697153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA86150.075.3479461
TAGTAAC98700.065.417032
ACAACGC37300.065.026878
GCCGACC11000.060.63516145
AACAACG41150.059.4543657
AAGAGCG18200.055.2324529
CAACGCA60750.053.4068839
AGTAACA124550.051.8935243
TGACGCC22600.051.8802145
TAACAAG128550.050.326455
ACACGAG42100.049.740587
AAACACG65200.049.0824135
GTAACAA131650.048.9325834
GACGCTC5100.048.182735145
ACGAGCC19150.048.1482549
GTCGCCC13050.047.88209145
ACGATAA16200.046.806087145
CACGAGC44300.046.7736328
CAAGAGC135400.045.3981978
CGACGAA29100.045.358482145
Produced by FastQC (version 0.11.5)