FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_67_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_67_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235762
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA27020.21865051684709516No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA19910.16111516618895871No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA13570.1098107888088483No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT13510.10932525842354758No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC13000.10519825014849138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10800.091.579291
TAGTAAC32700.089.470032
GCACGCA11300.088.139192
GTAGTAA33950.086.58331
AACACGT6650.086.294536
CGCACTT13050.078.956965
AAACACG19700.077.929415
GTAACAA39800.073.1528554
AGTAACA40000.072.790043
ACGCACT14750.072.6728064
CACGCAC13800.071.664953
TAACAAG41000.071.008925
GAAACAC25000.062.240744
GCCGACC1450.058.119637145
ACGAGCC8000.058.0971649
AACACGA17150.055.634196
ACACGAG17500.054.5215077
CGGGTAC651.3207633E-554.021454145
ACACGTG10950.053.6700027
GACGAAA5250.053.50696145
Produced by FastQC (version 0.11.5)