FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_66_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_66_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093360
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT35040.3204799882929685No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC34740.31773615277676154No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT28540.26103021877515187No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT28250.25837784444281847No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT24010.2195983024804273No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23580.21566547157386406No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21660.19810492427013976No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG19130.17496524475012804No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT18780.1717641033145533No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT16670.15246579351723127No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC16060.14688666130094388No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTC14470.1323443330650472No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG14010.12813711860686325No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13140.12017999560986319No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC13110.11990561205824249No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12940.1183507719323919No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT12740.11652154825492061No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA12520.11450940220970221No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT12310.11258871734835735No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12060.11030218775151825No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC12040.11011926538377113No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT12020.10993634301602401No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11620.10627789566108144No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT11610.10618643447720788No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG11470.10490597790297797No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG11370.10399136606424234No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA11220.10261944830613887No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA11160.1020706812028975No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG11070.10124753054803542No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT10970.10033291870929976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG100.007801196140.44305145
TGCGTTA100.008186834138.194412
GTAACAA23750.0115.490874
GAAACAC5750.0109.3438344
GAGCAGA2150.0109.270011
AGCACGC3800.0107.282521
ATAAGCC204.3897287E-4105.33229145
TAACAAG26150.0104.627125
AAACACG6200.0103.636345
GCACGCA4100.099.432572
CACGCAC4150.098.234583
TTCGGAA451.3627869E-893.6287145
GACCCCC601.0913936E-1193.62869145
AACACGT1550.084.676576
GCCACCC250.001065631384.26583145
TAGTAAC34200.082.4317552
GGAGCGT601.2169039E-980.591296
GCCGACC900.078.02392145
TGACGCC1750.076.24051145
AGTAACA37250.075.682323
Produced by FastQC (version 0.11.5)