FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_65_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_65_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076983
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22170.20585283147459152No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT21140.19628907791487885No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT20830.19341066664933432No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA20310.18858236388132404No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG14550.13509962552797955No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13840.12850713521011936No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA13440.1247930561578038No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT13010.12080042117656453No Hit
CCTTTAATAGTTTTGCAGGAGGTCTATATTTGGTTCCATTGGCAAGCTTC11700.10863681228023099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC9200.0107.5298841
GCACGCA9450.0104.685182
GTAACAA35500.094.292464
CGCACTT10850.091.789445
CACGCAC10950.090.336363
TAGTAAC38800.086.475082
TAACAAG39350.085.242655
AAACACG12750.084.620275
ACGCACT12450.079.99324
GTAGTAA42750.078.970471
AGTAACA42900.078.20333
GAAACAC14250.077.1688844
GCTATCG300.002334725369.154377
AACACGT4300.069.1543666
GTCTCGC6150.067.492751
CTCGCTC6250.066.40673
ACAAGAG51900.064.757277
CGCTCTG6550.062.2975125
AACAAGA55650.060.3935746
CAAGAGC47250.060.1661878
Produced by FastQC (version 0.11.5)