FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_63_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_63_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933942
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG16910.18106049412061992No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16060.17195928655098497No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC12020.12870178233766122No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA10590.11339033901462832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4100.074.260512
AGCACGC4200.072.492411
AAACACG6900.069.182485
GTAACAA21150.066.40214
CACGCAC4750.064.0951163
TAACAAG22600.062.141785
AACACGA5600.061.763466
TAGTAAC23450.061.3775632
GACGAAA5300.058.235893145
ACAACGC4150.056.847048
ACACGAG6350.054.4685637
CACGAGC6150.054.155578
TATCGGG400.00689386752.61084145
GTAGTAA29150.051.5122261
AGTAACA27700.051.4580543
AACACGT2700.051.2407956
CGCACTT6750.049.196435
ACGTGCA3600.048.053682
TGACGCC2800.047.60028145
GAAGCGG3250.046.841251
Produced by FastQC (version 0.11.5)