FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_61_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_61_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729670
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17470.23942330094426245No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11310.15500157605492895No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG10360.14198199185933366No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC8830.12101360889169076No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT8020.10991270026176217No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA7660.10497896309290501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC4750.087.3542941
CGGAGCG408.107454E-786.43255
GCACGCA4950.085.221912
ACGGCGA250.001119035283.231898
GAAACAC6400.079.9500664
AAACACG6600.077.527345
GTAACAA17900.075.3266454
AACACGA5400.070.426496
GCCGACC801.3278623E-1070.180046145
ACACGAG5450.069.780377
CACGCAC6050.069.722243
TAGTAAC20500.067.1314242
GACGAAA3800.064.63952145
GTAGTAA22450.064.0727841
TAACAAG21050.064.05455
CGCACTT7200.061.4631165
AACACGT2200.059.7176
AGTAACA23450.058.977183
ACGCACT7800.058.5081564
CACGAGC6650.058.4083378
Produced by FastQC (version 0.11.5)