FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_5_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_5_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793392
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA12770.16095448403815515No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC11810.14885453848791014No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11590.1460816342993123No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT11390.14356081230967793No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11020.13889729162885434No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG10860.13688063403714684No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT10310.12994837356565228No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC10250.12919212696876198No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG9920.12503277068586524No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT9750.12289007199467603No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT9540.12024320890555992No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA9350.11784842801540728No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8610.10852138665376006No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8520.10738701675842459No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT8470.106756811261016No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT8310.10474015366930849No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC8240.10385786597293645No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA8090.10196724948071066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC100.0077977744140.4612145
AAACACG4550.085.037355
AACACGT2000.082.900966
ACACGAG2800.078.953317
GAAACAC4950.078.165654
CTTACGG451.4892157E-678.034004145
AACACGA2900.076.230786
ACACGTG2200.075.364527
ACGAGCC1200.075.1157469
CACGAGC2950.070.481618
AGACGCC300.002195807970.230606145
TAGTAAC9900.069.101562
GAAGCGG2550.067.746631
GTAACAA10250.066.733594
AAGCGGA2650.065.1901552
GCCGACC2000.063.20754145
TGACGCC2900.062.96537145
TAACAAG11650.058.7140965
ACGTGCA1900.054.7402159
GTCGCCC2200.054.2691145
Produced by FastQC (version 0.11.5)