FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_57_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_57_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436834
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28130.19577766116336334No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20050.13954291170726751No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18130.12618019896522495No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17690.12311791062850684No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC17010.11838528319903342No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC16980.11817649081243903No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA16870.1174109187282595No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT16100.11205191413900283No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT15030.10460498568380203No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA14530.1011251125738951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA26550.089.069474
CGACTAA407.4072705E-787.76157145
ACGAGCG407.9630263E-786.701179
TAACAAG29350.080.328245
AAACACG9550.078.912365
AGCACGC3900.070.9313661
GCCGACC1300.070.20926145
GCACGCA4000.069.158082
GAAGCGG4700.066.215181
AACACGT3750.064.518336
TAGTAAC37200.064.510362
GAAACAC11800.064.4581764
AACACGA8150.062.7654276
ACACGAG8500.060.180977
AGTAACA40500.059.422653
CACGCAC5000.058.090763
AAGCGGA5450.057.1032
TGACGCC2850.056.66011145
GACGAAA7000.055.16442145
ACACGTG4500.053.765287
Produced by FastQC (version 0.11.5)